Sentence examples for minute before application from inspiring English sources
The periosteum was stripped from the surface of the tibia cortical bone 1 minute before application of the assigned RFE.
One minute before application of focused ESWT, baseline measurements were carried out on the dorsal lower leg of the hind limb of each animal.
For DAB chromogenic staining technique, tissue was incubated in 3% H202 in methanol for 10 minutes before application of primary antibody.
Slides were counterstained with Hoechst 33258 blue (10 μM in PBS; Sigma) for 5 minutes before application of coverslips that were secured with a sealant.
The material consists of highly osteoconductive hydroxyapatite particles embedded in a calcium sulphate (CaS) paste, which is prepared 3 minutes before application by mixing with the water-soluble radiocontrast agent, iohexol, in a closed system.
Surgery was performed under pneumatic tourniquet control and antibiotic prophylaxis using intravenous Cefazoline 1 g, 3 times daily, for the first 24 hours with the first dose administered 30 minutes before application of the tourniquet.
In brief, cut sections were subjected to on-board dewaxing (Dewax solution; Vision BioSystems) and antigen retrieval (Epitope Retrieval 1 solution; Vision BioSystems) for 20 minutes before application of primary antibody (1 200) and detection by using the Bond polymer Refine detection system (Vision BioSystems).
Be sure to apply facial lotion about thirty minutes before application to avoid serious breakouts.
In striking contrast, the same concentration of H2O2 was completely ineffective in potentiating [3H]taurine release in synaptosomes, whether H2O2 added 10 minutes before and during application of hypoosmotic medium (Fig. 9b) or acutely (data not shown).
After this, for direct comparison of the activations in the different conditions, it was determined per neuron whether the activity after ACh application was higher, lower, or equal to the amount of calcium events in the minute before ACh application.
Cytochalasin D (30 nM; Sigma) was added 30 minutes before dye application and confocal imaging.
