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With dissecting microscopy we counted their sexual branches (♂ perigonia, ♀ perichaetia and bisexual branches in some species).
Then, using transmission electron microscopy we counted the number of RPE lipofuscin granules.
Using inverted microscopy, we counted the number of colonies containing 12 or more tumor cells in each well.
Using fluorescence microscopy, we counted GFP-expressing cells and those with CD45 immunofluorescence were judged to be WBCs.
The MCs were identified and counted in three different ways: (a) light microscopy: we counted the MCs with a hemocytometer and used trypan blue to detect any dead cells [ 30]; (b) flow cytometry: BMMCs were stained with FITC rat anti-mouse CD117 antibody, and the percentage of MCs was analyzed with flow cytometry.
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Unlike the studies of Wilson et al. and Maier et al., who identified different generations of myogenic cells using electron microscopy [ 9, 11], we counted the muscle fibers under a light microscope and found many small-diameter fibers until 135 d in both breeds.
To determine whether lysosomes were increased in these sheep, we performed cathepsin immunolabeling at light microscopy and counted endosomal-lysosomal structures by electron microscopy.
We selected representative samples for each treatment, examined them with light microscopy, and counted cells to verify that reduced DNA content connoted fewer cells.
Apoptotic cells were labeled using a TUNEL assay kit according to the manufacturer's instructions (Roche, Nutley, NJ), imaged using an epifluorescence microscopy, and counted.
All characteristic colonies were checked by microscopy and counted (Arla Foods amba).
TUNEL-positive cells were visualized by immunofluorescent microscopy and counted using a 20× objective.
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