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Using epifluorescence microscopy, we detected individual, freely moving filaments in solution in the absence of sAB-27 (Fig. 3a).
Performing confocal laser scanning microscopy, we detected E-cadherin in the plasma membrane of uninfected MCF-7 cells (Figure 4B and 4C, 'mock'mock
Using electron microscopy, we detected iron nanoparticles in the cytoplasmic compartment, enclosed within endosomes, suggesting that these particles are biologically inactive and cause little or no interference with cell physiology.
Using fluorescence microscopy we detected delivery of the YopK-βlac fusion protein into HeLa cells infected with a yopK null mutant (Figure 1E, upper right), but we did not detect delivery of the fusion protein into HeLa cells infected with the translocation-deficient yopB mutant (Figure 1E, upper left).
By light microscopy, we detected no apparent differences between E. bangladeshi and E. histolytica.
Using immunofluorescence microscopy, we detected reduced colocalization of TLR9 and the lysosomal marker Lamp-1 in UNC93B1-Y539A-expressing cells.
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Following immuno-electron microscopy we detect SmAQP by immunogold labeling and observe gold particles often clustered in the parasite tegument.
In this regard, using transmission electron microscopy (TEM) we detected XMRV VLP in HeLa cells infected with Ad5-XMRV (Fig. 2C, Panels I and II).
Using confocal microscopy, we also detected that the number of IL-17-producing CD4+ T cells was significantly higher in minor salivary glands of patients with SS than in disease controls.
By immunofluorescence microscopy, we could detect the presence of CYP27B1 in bladder tissue sections (Figure 3B), localized throughout the cytoplasm with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria [14].
By combining whole-cell recording with confocal microscopy we could detect Ca2+ entry into individual spines.
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