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Using super resolution microscopy, we identified co-clustering of the active zone scaffolding proteins bassoon, RIM and ELKS in ∼30% of dopamine varicosities.
Whereas the functional improvements by PPADS were persistent at day 28, the infarct volume measured by magnetic resonance imaging and the amount of TUNEL-positive cells were significantly reduced by PPADS only until day 7. Further, by immunohistochemistry and confocal laser scanning microscopy, we identified both neurons and astrocytes as TUNEL-positive after MCAO.
Using confocal microscopy, we identified the expression of Bcl-2 at the single FLS level.
Using electron microscopy, we identified numerous areas containing abnormal clusters of sarcomeric material (Fig. 5A).
Using confocal microscopy, we identified the expression of IDO in situ at the single-cell level.
On the basis of the above characteristics, and especially taking into account the ascospore ornamentation observed under scanning electron microscopy, we identified the isolate as N. hiratsukae (10, 11).
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By constructing a series of deletion mutants fused with EYFP and fluorescence microscopy analysis, we identified an N-terminal arginine-rich functional NLS (PASTPTPPKR GRYVVEHPEY) in P-ICP22, and KR is the crucial aa for its nuclear localization.
Utilizing whole LN 3D imaging, histo-cytometry, and intravital 2-photon microscopy, we have identified a specialized population of DCs, enriched in the LN-resident CD11b+ subset, which resides within the lymphatic sinus endothelium and scans lymph with motile dendrites.
Overall, using a combination of proteomics, bioinformatics and microscopy we have identified subunits of Drosophila NDC80 (dmNuf2R, dmSpc25, dMIND/MIS12INdmNsl1R (dmMis12, dmNsl1R, dmNnf1R-1 and dmNnf1R-2) and SPC105 (dmSpcomplexesmplexes.
Using live-cell phase contrast microscopy, we occasionally identified large, distorted structures resembling mitotic profiles (data not shown), but none were observed in normal telophase/cytokinesis.
Using a combination of laser-scanning confocal microscopy and atomic force microscopy, we have identified flexible, actin-based structures on the surface of cells derived from the vertical growth phase of melanoma progression.
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Justyna Jupowicz-Kozak
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