Sentence examples for microscopy we fixed from inspiring English sources

For electron microscopy, we fixed the dissected PSGs and processed the samples as previously reported [55].

For light microscopy, we fixed 10 ml of a growing yeast culture by adding 1.34 ml of formaldehyde stock solution (50 mM K-Pi buffer, pH 6.8; 0.5 mM MgCl2; 4.8% formaldehyde) and incubating for a further hour at 28°C, with shaking at 250 rpm.

To test this possibility, we performed electron microscopy of fixed optic axons of NF-L null and DL20J mice, and further morphometric analysis by counting clear ER vesicles in these mutant axons and calculating the density of ER (see methods).

Whole-granule recapture within intact islets has been controversial and to further our analysis we performed electron microscopy on fixed islets.

Specimens for Electron Microscopy were fixed in Karnovsky fixative, postfixed in 1% phosphate-buffered osmium tetroxide, and embedded in standard fashion.

On the other hand, from both time-lapse microscopy and PFA fixed samples, we did not see apparent defects in chromosome alignment between cells expressing GFP-WT and GFP-5R, indicating that Kif18A sumoylation does not regulate the capture of microtubules to the kinetochores and the movement of chromosomes during congression.

Slices used for confocal microscopy were fixed and tyrosine hydroxylase was immunostained (Fig. 1A).

To identify the spatial origins of fluorescent signals accurately, the same frozen section slides used for microscopy were fixed and histochemically stained immediately following LSCAM imaging.

Material for scanning electron microscopy was fixed and examined as explained previously [43].

Cells grown on 22-mm2 cover slips for conventional fluorescence microscopy were fixed with 4% paraformaldehyde for 12 h at 4°C.

Samples for electron microscopy were fixed in cacodylate-buffered glutaraldehyde (4.5%, pH 7.3), postfixed in osmium tetroxide (1%) and embedded in Spurr's resin.