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TEFAP analysis using the primer pairs which produced diverse microbial sequences in the current study represents a feasible, albeit limited approach for detecting cryptic bacterial and ascomycete endophytes within plant matrices.

These results perhaps provide a line of preliminary evidence for the presence of microbial sequences in these lepidopteran genomes, possibly by horizontal transfer.

Here, we report an approach developed on the basis of DK and computational subtraction for the identification of microbial sequences in human tissues in any state.

Our investigation confirmed the presence of microbial sequences in 31 (24%) of 131 CSF samples; 25 were identified by MassTag PCR.

The NGS-based pathogen detection is a new-developing method for scanning the microbial sequences in clinical samples, by which we can easily identify potential pathogens for performing specific antimicrobial treatment.

After computational subtraction of human host reads, alignment was performed against all microbial sequences in the National Center for Biotechnology Information nucleotide database and the best hit selected on the basis of percentage of mapped read coverage and pairwise identity.

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We also conducted BLAT searches on microbial databases as a filter to remove possible contamination as well as endogenous microbial sequences found in the elephant placenta.

In patients with chronic RTIs, the defending frontier retreats from the throat to the trachea or bronchia as indicated by increased number of microbial sequences found in bronchoalveolar lavage fluid (BALF) and sputum samples [27,29].

Candidate microbial sequences are summarized in Table 6.

DNA microarrays are able to detect microbial sequences from any sample in a parallel and very fast, high throughput way.

DNA microarray development enabled researchers to detect microbial sequences from any sample in a parallel and very fast high throughput manner.

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