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While collaborating with Lewis on a device to sequester microbial colonies in nature, he became the chief tinkerer.
We anticipate that our approach will be a starting point for elaborate studies of the population dynamics, evolution, and ecology of microbial colonies in complex landscapes.
Additional file 6: Readme file for the 3D MALDI imaging MS dataset of microbial colonies in a time course experiment.
Additional file 12: Script for the 3D MALDI imaging MS dataset of microbial colonies in a time course experiment.
A visualization of the 3D dataset of the microbial colonies in a time-course experiment is shown in Additional file 2: Figure S4.
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There was no microbial colony in any of the plates for sterility check of high-dose PHEA-C16-iron oxide and S-9 mix.
Allen et al. have developed a mathematical model of a public goods dilemma within a microbial colony, in which the public good travels from its producers to other cells by diffusion.
3D imaging MS data were collected from five samples using two types of 3D imaging MS. 3D matrix-assisted laser desorption/ionization imaging (MALDI) MS data were collected from murine pancreas, murine kidney, human oral squamous cell carcinoma, and interacting microbial colonies cultured in Petri dishes.
The plating of samples on agar plates and manually counting microbial colonies, while standard in immunological research, is time consuming, requires comparatively large amounts of samples, and can be less reliable.
In the following, we estimate under which conditions clonal interference might be observable in microbial colonies and discuss our key predictions and how they may be verified in microbial evolution experiments.
A particularly interesting aspect of these sediments is the distribution of the microbial colonies and the way in which their traces were preserved.
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