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Exact(29)
We identified 14 most abundant phyla (represented by more than 1 % total bacterial sequences) in the freshwater sponges and its surrounding water (Fig. 1).
To this end, we selected the bacterial sequences in the complete STAP database which had more than six levels of taxonomic annotation.
There is however, a significant bias towards bacterial sequences in the RAST and MG-RAST database for comparison, hence all hits identified below were explored to make sure that if the RAST homologue was bacterial, that there was the potential for a eukaryotic homologue in the NCBI nr database.
Sequences of the isolates (~800 base pairs in length) were compared to known bacterial sequences in Genbank by BLAST algorithm.
The effective bacterial sequences in the four samples were all assigned to corresponding taxonomies by using BLAST combined with MEGAN.
By contrast, the majority of bacterial sequences in the wallaby gut belong to the Firmicutes phylum, similarly to what is observed in human and mouse guts.
Similar(31)
A single bacterial sequence in the midgut transcriptome showed similarity with a Wolbachia sequence, an ubiquitous endosymbiont found across various insect taxa and nematodes [62] [65].
None of the genera represented in this survey matched genera found in a previous 16S survey of bacteria from adult honey bees [ 8], suggesting that bacterial sequencing in bees will continue to identify novel taxa.
This level of coverage indicated that the 16S rRNA sequences identified in these groups represent the majority of bacterial sequences present in the samples under study.
Phylogenetic affiliations for each sequenced DNA band were assigned by comparison to known bacterial sequences recorded in the National Center for Biotechnology Information database [GenBank, [47]].
The obtained sequences were aligned and compared with the bacterial sequences available in the GenBank database.
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