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Salmonella microarray slide preparation and general microarray analysis followed the procedures described by Porwollik et al. [63].
Our microarray analysis followed by functional annotation clustering revealed statistically significant enrichment related to metabolism and membrane transport and transporters.
RNA cleanup was carried out on samples prior to microarray analysis, followed by assessment of RNA yield and purity (full details available on request).
The purpose of the present study is to examine the phytoestrogenic effect of SWT using the whole human genome microarray analysis followed by pharmacological studies.
Samples from Batch 2 (n=14) were used for microarray analysis followed by quantitative real-time PCR (qPCR) on selected genes.
We prepared cDNA from the total RNA extracted for microarray analysis, followed by DNase treatment and amplification by PCR as described by Pastore et al. (2011) [ 20].
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Microarray analysis was followed by comprehensive bioinformatics and several levels of experimental validation.
From the initial microarray analysis we followed up gene-expression changes by qRT-PCR analysis in SOX9-SW1353 cells to confirm their regulation.
Gene expression microarray analysis was followed by in vitro validation and cell migration assays to elucidate the downstream molecular pathways involved in this process.
Using a new microarray analysis tool followed by a posteriori experimental validation of the results, we have confirmed several putative markers of malignancy associated with peptide growth factor signalling in prostate cancer and revealed others, most notably ERRB3 (HERRB3
Synthesis and labeling of cDNA as well as DNA microarray hybridization, signal detection and data analysis followed protocols described previously [ 23].
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microarray analysis allowed
microarray analysis observed
microarray design followed
microarray hybridization followed
microarray chip followed
microarray slide followed
microarray analysis detected
microarray preparation followed
microarray technology followed
microarray experiment followed
microarray platform followed
microarray approach followed
microarray study followed
microarray dataset followed
microarray technique followed
microarray analysis showed
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