Exact(1)
Previous work has shown that the experimental FDR for a porcine brain microarray platform followed statistical expectations without the addition of a fold cut-off and that additional selection criteria could be used to virtually eliminate false positives [ 29].
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Briefly, the protocol for the Agilent microarray platform is as follows: RNA samples are labelled with Cy3 and Cy5 fluorescent dyes using a Low RNA Input Fluorescent Linear Amp Kit (Agilent Technologies, Wilmington, DE) following the manufacturer's instructions.
The Living Microarray platform can be applied to many systems.
The correlation coefficients and linear slopes for the comparison between PCR Arrays and different microarray platforms are as follows: 0.95 and 0.62, respectively, for AFX; 0.94 and 0.78, respectively, for AG1; 0.90 and 0.61, respectively, for ABI; 0.86 and 0.56, respectively, for GE H and 0.92 and 0.60, respectively, for ILM.
We performed an outlier analysis and were able to detect the highest degree of disagreement (comparing each microarray platform to the rest) in Operon, followed by Illumina and Agilent (Data not shown).
Following NCBI Gene Expression Omnibus GEOO) database search, microarray platform validation, differential expressions of REGγ in corresponding cancers were statistically analyzed.
We termed this combination of MBD2b/MBD3L1 enrichment [ 5] of DNA followed by KLM-PCR and subsequent hybridization to a microarray platform MeKL-chip.
The accumulated transcribed sequences could be directly used to develop microarray platform.
Human microarray platform, GPL1528; human HCC microarray data, GSE1898; mouse microarray platform, GPL1529; mouse HCC microarray data, GSE1897.
A robust small-molecule microarray platform for screening cell lysates.
We generated the data from each mouse model using the same microarray platform and reference RNA.
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