Sentence examples for microarray hybridization followed from inspiring English sources

These results indicate that the combination of in vitro microarray hybridization followed by Q-PCR validation is a good tool for discovering genes that may act as biomarkers, as well as for investigating the early stages of environmental diseases.

Differential expression of organ-specific sunflower sequences previously obtained by suppression subtractive hybridisation (SSH) [ 50] was evaluated with regard to the response to chilling and salt stresses using cDNA fluorescence microarray hybridization followed by Northern blot and qRT-PCR validation.

She also constructed the substractive library, PCR amplified and purified the 5760 library clones for the array chips, conducted the microarray hybridizations followed by data analysis and sequencing of the clones of interest.

For isolation of RNA from large numbers of cultured cells, standard RNA extraction and microarrays hybridization we followed standard protocols as described elsewhere [ 6, 16].

Following microarray hybridization, all arrays were assessed for quality using a set of standard metrics (including RNA degradation), as implemented in the AffyQCReport library in R; all arrays passed quality control checks.

Following microarray hybridization and analysis, 86.7% of all coding sequences included on AppChip2 could be detected during our experiments, and 150 genes were significantly differentially expressed in vivo when their level of expression was compared to that seen in vitro, with a FDR of 4.25% over the course of three hybridizations: 72 of these were up-regulated, while 78 were down-regulated.

The cDNA synthesis and in vitro transcription amplification were followed by microarray hybridization using the Human HT-12 v.4.0 Expression BeadChip kit following the manufacturer recommended protocols (Illumina).

Chromatin Immunoprecipitation (ChIP) followed by microarray hybridization on the whole genome tiling arrays (ChIP-chip; Iyer et al., 2001; Ren et al., 2000) or followed by massively parallel DNA sequencing (ChIP-seq) (Johnson et al., 2007), are now established as powerful methods to identify all of the genomic regions bound by a protein of interest in a given condition (Keles, 2007).

One commonly used approach to detect regulatory interactions between TFs and genes is chromatin immunoprecipitation followed by microarray hybridization (ChIP-chip)[1], [2], which is a binding assay.

We have mapped SALL4 global gene targets using chromatin-immunoprecipitation followed by microarray hybridization (ChIP-on-chip) in myeloid leukemic NB4 cells [28], normal human CD34+ bone marrow cells, and 293 cells (unpublished data).

To determine the molecular mechanism(s) of SALL4 in normal hematopoiesis, leukemogenesis and ESC development, we have mapped SALL4 global gene targets using chromatin-immunoprecipitation followed by microarray hybridization (ChIP-on-chip) in normal human CD34+ bone marrow cells, myeloid leukemic NB4 cells and human ESCs.