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For tight confinement of erythrocytes in the microchambers, erythrocytes in RPMI 1640 medium at a hematocrit of 0.25%, 0.5%, 0.75%, or 1.0% were dispersed on a cell microarray chip, followed by 10 min standing to allow the erythrocytes to settle down into the microchambers under gravitational force.
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Genome-wide location analysis, or ChIP-CHIP analysis (chromatin immunoprecipitation (ChIP) followed by DNA microarrays (CHIP)) allows the identification of direct targets of a defined TF on a genomic scale [ 14].
TSS: transcription start site; ChIP-chip: Chromatin Immunoprecipitation (ChIP) followed by microarray analysis; E2: 17β-estradiol; CAGE: cap analysis gene expression; RNA Pol II: RNA polymerase II.
Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone modifications.
Modern high-throughput techniques, such as chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or by massively parallel sequencing (ChIP-Seq), allow genome-scale mapping of TF occupancy in a given cell type and state [ 1].
Chromatin immunoprecipitation (ChIP) followed by genomic tiling microarray hybridization (ChIP-chip) or massively parallel sequencing (ChIP-seq) are two of the most widely used approaches for genome-wide identification and characterization of in vivo protein-DNA interactions.
Chromatin Immunoprecipitation (ChIP) followed by microarray hybridization on the whole genome tiling arrays (ChIP-chip; Iyer et al., 2001; Ren et al., 2000) or followed by massively parallel DNA sequencing (ChIP-seq) (Johnson et al., 2007), are now established as powerful methods to identify all of the genomic regions bound by a protein of interest in a given condition (Keles, 2007).
Chromatin immunoprecipitation(ChIP) followed by microarray (ChIP-chip) [ 3] or sequencing (ChIP-seq) [ 4] has been extensively used to study the in vivo binding locations of individual transcription factors and cofactors in a wide range of species and tissues [ 1, 2, 5- 9].
TGF-β: transforming growth factor beta; SBE: SMAD Binding Element; TFBS: transcription factor binding site; ChIP-chip: Chromatin Immunoprecipitation (ChIP) followed by microarray analysis; CART: Classification And Regression Tree Analysis; RF: Random Forest algorithm; IOSE: immortalized ovarian surface epithelial cells The authors declare that they have no competing interests.
They were considered to be new miRNAs and named according to the probe ID on the microarray chips as follows: hsa-miR-27b-3p, hsv1-miR-H6-5p, hsa-miR-1265 hsa-miR-1265 hsa-miR-12650, hsa-miR-9448-3p, hsa-miR-39605p, and hsa-miR-4708-3pable 2, Supplementary Table S5).
ChIP-seq allows the identification of binding sites of chromosome-interacting proteins with high resolution, compared to ChIP-chip followed by microarray analysis (Schmidt et al. 2009b).
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