Sentence examples for membrane were incubated from inspiring English sources

These petri dishes either containing samples of water or membrane were incubated at 30 °C for 3 days for possible microbial growth.

Strips of this membrane were incubated either with conditioned medium containing the recombinant C. elegans agrin fragment or with purified recombinant chicken agrin fragments representing the muscle (A0B0) and neuronal (A4B8) agrin isoforms.

Those pieces of membrane were incubated with individual serum containing autoantibody against 90 kDa autoantigen for 2 h at RT.

Second, the membrane were incubated with an alkaline phosphatase-linked secondary antibody (donkey anti-mouse) for 1 h and washed 3 times with TBST (15 min each time).

Cells that had migrated through the polycarbonate membrane were incubated with cell stain solution (kit components), then subsequently extracted and detected on a standard microplate reader (Benchmark Plus microplate spectrophotometer, Bio-RAD, USA), at 560 nm.

Then, rSEPs on the PVDF membrane were incubated with the acute- or convalescent-phase serum (1 250 dilution) that was previously neutralized with E. coli lysate (5 mg/ml) for 1 h.

After another washing step, the membrane was incubated with secondary antibodies (Beijing CWBIO Co., Ltd).

The nitrocellulose membrane was incubated with EhAACT antibodies (diluted 1/1000 with TBST) overnight at 4 °C.

After washing, the membrane was incubated with anti-mouse or anti-rabbit peroxidase-conjugated secondary antibodies (ZSGB-Bio http://www.zsbio.com/).

For hybridization, the membrane was incubated at 65℃ o/n in 10 ml Southern blot hybridization buffer.

The membrane was incubated with GSK3β, and bound GSK3β was detected with an anti-GSK3β antibody.