A novel integration between the amine capture plant and the selective membrane is analysed here, which aims at exploiting the benefits of both the parallel and series selective exhaust gas recirculation (S-EGR) existing options.
The two-stage integral sorption data for water vapour in a glassy poly 2-hydroxyethyl methacrylate) (poly 2-hydroxyethyl analysed by the variable-surface concentration methacrylateiffusion-relaxation model, and the diffusion-reaction model.
The membrane was analysed using indirect end-labelling with a probe generated by random primer labelling from a 151 bp PCR product prepared using the primers 5'-tgtgcaaattccaactaaagga-3' and 5'-ggcgataatttatcatgttttcc-3'.
The NPSR1 expression on the plasma membrane was analysed with BD FACSCalibur™.
To quantitate the amount of DNA transferred, the membrane was analysed using the Storm 840 phosphorimaging system (Molecular Dynamics, Bucks, UK).
Plates were incubated at 37°C in 5% CO2 and the number of cells migrated to the lower side of the membrane was analysed 24 hours later.
The invasion capacity from serum free towards serum containing medium through extracellular matrix gel and 8 µm pore membrane was analysed using the Millipore Colorimetric Migration Assay on 24-well plates (BD Falcon, Franklin Lakes, NJ, USA).
The translocation of phosphatidylserine from the inner part of the plasma membrane to the outer layer was detected with Annexin-V-FLUOS staining; the disruption of the cytoplasmic membrane was analysed by the formulation of PI DNA complexes by flow cytometry with a FAC Sort FCM machine (Beckman Coulter, Fullerton, CA, USA).
