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Signals on the PVDF membrane were visualized with an enhanced chemiluminescence kit.
After three washes in TBST, Proteins on the membrane were visualized using the enhanced chemiluminescence detection system (Amersham, Arlington Heights, IL).
The bands on the membrane were visualized using enhanced chemiluminescence method (Pierce Biotechnologies Inc .. Densitometric analysis was performed using a Bio-Rad image acquisition system Bio-Rad Laboratoriess). Bio-Rad Laboratories
Target proteins on the membrane were visualized on X-ray films by using the ECL Plus Western Blotting Detection Reagents (Amersham, Buckinghamshire, UK).
The proteins on the membrane were visualized using a CCD digital camera, as described below.
The bands in the membrane were visualized and analyzed by Imagelab software.
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However, a floating intimal membrane was visualized consistent with aortic dissection, Fig. 1.
The membrane was visualized with ECL Western Blotting Detection Reagent Pierce Biotechnologyy).
The membrane was visualized with ECL Western Blotting detection reagents (Amersham Biosciences, Piscataway, NJ).
After extensive washing in TBS/Tween over more than one hour, protein on the membrane was visualized by ECL reagent (Amersham Biosciences) and exposed on Kodak Biomax MR film.
After a further 3 washes, the membrane was visualized enhanced chemiluminescence reagents (Thermo scientific) For a loading control, the monoclonal Anti-β-actin antibody (Sigma Aldrich) in a dilution of 1∶2000 against the β-actin protein was used.
More suggestions(15)
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