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Exact(14)
The non-migrated cells from the upper side of the membrane were scraped off gently with a cotton bud.
The non-migrant cells remaining on the upper surface of the filter membrane were scraped off gently with a cotton swab.
Two days later, membranes were fixed in 4% PFA and the cells on top of each membrane were scraped off.
Then cells on the top surface of the membrane were scraped with a cotton swab.
Cells on the upper part of the membrane were scraped using a cotton swab.
Non-migratory cells on the upper side of the membrane were scraped off with wet cotton swabs.
Similar(46)
After 7 days incubation, the surface of the cultured membrane was scraped and Gram staining was performed.
Cells remaining on the upper face of the membranes were scraped, and those on the lower face were counted using an inverted microscope.
After filtration, the membranes were scraped, and the collected material was washed with 100 mL eluted fluid consisting of phosphate-buffered saline (PBS; 7.5 mM Na2HPO4, 3.3 mM NaH2PO4, and 108 mM NaCl, pH 7.2), 1% Tween 80, and 1% sodium dodecyl sulfate.
Cells remaining on the upper surface of the filter membrane (non-migrant) were scraped off gently with a cotton swab.
For membrane binding experiments, confluent cells were scraped off 75 cultureture plates into ice-cold lysis buffer (20 mM Tris-HCl, pH 7.0, 5 mM ethylenediaminetetraacetic acid (EDTA), and 1× protease inhibitor cocktail (Roche Diagnostics, Ltd.,West Sussex, UK).
More suggestions(15)
membrane were analysed
membrane were visualized
membrane were placed
membrane were removed
membrane were investigated
membrane were tested
membrane were formed
membrane were analyzed
membrane were autoclaved
membrane was scraped
membrane were seen
membrane were counted
membrane were used
membrane were recorded
membrane were revealed
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