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Exact(7)
PDGF-BB pre-treatment of neurons resulted in the maintenance of mitochondrial membrane potential even in the presence of Tat and morphine as indicated by JC-1 aggregates that appeared red.
Using live imaging techniques we show that PINK1 deficiency causes a reduction in the basal ψm in human neurons, implicating a role for PINK1 in the maintenance of membrane potential, even in the absence of stress.
We show that BK channels in LNCaP cells may open at resting membrane potential, even in conditions of low cytosolic Ca2+ concentrations.
Indeed, Garlid has argued convincingly that the fluxes of K+ predicted to occur if the channel opens would allow minimal perturbation of the membrane potential even when giving a significant increase in matrix volume [169,229].
On the other hand, addition of dithiothreitol together with idebenone did not cause PTP opening and allowed maintenance of the mitochondrial membrane potential even in the presence of rotenone.
The method described here is useful for investigating mitochondrial heterogeneity and assessment of membrane potential, even if many mitochondria are damaged and depolarized during the preparation and separation, which would not be possible using bulk techniques.
Similar(53)
Subunits γ1 (LRRC26), γ2 (LRRC52), γ3 (LRRC55), and γ4 (LRRC38) produce a shift towards more hyperpolarized membrane potentials, even in the absence of intracellular calcium and transcripts for all four subunits are detectable in human testis (Yan and Aldrich, 2010, 2012; Yang et al., 2011).
As now stated in the Results section, saponin was added "to avoid the potentially confounding influence of variations in plasma membrane potential", because even small variations in the plasma membrane potential caused by the potassium leak may change the interpretation of the data.
Importantly, this loss of membrane potential occurs even when the chimeric proteins are exogenously expressed at the lowest levels detectable using this method (Fig. 5A).
Don't get stuck on the HH model itself, or any other deterministic model of the processes that affect membrane potential--even though deterministic models are generally "good enough" if channel densities are high enough.
B. Surprisingly, the amplitude of the EPSP depended upon the membrane potential of the presynaptic neuron, even though the same number and rate of action potentials were generated!
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com