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CL4 cells also have the lowest mitochondrial mass and membrane potential, though compared to CL1 cells their decrease is larger than predicted by our model (Additional file 2 Figure S4) (a detailed description of the results is given in the Additional file 2).
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Transient opening of Cx43 hemichannels that lie free in the plasma membrane, external to gap junctions, has been implicated in cell swelling, ATP release, and loss of membrane potential during ischaemia, though the presence of pannexin rather than connexin hemichannels has been suggested as an alternative explanation for such phenomena.
B. Surprisingly, the amplitude of the EPSP depended upon the membrane potential of the presynaptic neuron, even though the same number and rate of action potentials were generated!
Increased inward rectifier conductance lowered the resting membrane potential and lowered the cell excitability (though this is a weak effect), consistent with the literature (Doupnik et al., 1995).
Even though the mechanism controlling the mitochondrial membrane potential ∆Ψm in vivo are complex and not fully understood, 'mild un-coupling' of mitochondria are turned on in vivo to diminish the formation of ROS [38].
The average membrane potential was more depolarized in relation to control values, though not significantly so (Fig. 4A, n = 8, p = 0.15 paired t-test).
Their values can only be indirectly inferred through their influence on membrane potential or intracellular calcium (the main physiological quantities directly measurable though standard experimental techniques) and, in particular, on spiking activity.
ΔΨm; mitochondrial membrane potential.
Initial membrane potential = 0 mV.
TL; transmitted light, ΔΨm; mitochondrial membrane potential.
Additional analyses of mitochondrial membrane potential conduction.
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