Sentence examples for medium were dissociated from inspiring English sources

iNANOG cells and WTSG cells cultured with or without Dox, in either the E8 or 2iL/FK medium, were dissociated into single cells.

In brief, cells grown in normal culture medium were dissociated using TrpLE (GIBCO) or Cellstripper (Cellgro), washed once with serum-free Advanced D-MEM medium (GIBCO), re-suspended in the same medium, and finally counted with a hemocytometer.

Cells maintained in ITSFn medium were dissociated by 0.05% trypsin and 0.04% EDTA in PBS Biochrom AGG), neutralized with DMEM/F12 (1∶2) plus 10% FCS (Sigma-Aldrich), collected by centrifugation, and replated at a cell density of 0.5 2×105/cm2 on dishes precoated with polyornithine (15 mg/ml) and laminin (l µg/ml) (both Sigma-Aldrich).

Briefly, undifferentiated hESCs maintained on Matrigel in mTeSR1 medium were dissociated with Accutase solution.

Cells in certain volume of medium were dissociated by repetitive flushing through pipette with 200 ll tip.

Adhesive U251 cells cultured in serum were dissociated using a solution of 0.25% trypsogen containing 0.02% EDTA, whereas nonadhesive tumor spheres cultured in serum-free medium were dissociated with papain (1 mg/mL).

For naïve state conversion, cells cultured in standard hESC medium on MEFs were dissociated to single cells using 0.05% trypsin/EDTA solution (Invitrogen), replated on MEFs, and cultured overnight in standard hESC medium supplement with 10 μmol/L Rho Kinase (ROCK -inhibitor Y-27632 (Calbiochem).

After the ovaries had been chopped with forceps in TYH medium, tissue fragments were dissociated using a 21-gauge needle attached to a disposable 2.5 ml syringe.

Briefly, hippocampal neurons were dissociated using dissociation solution and cultured using the glial-conditioned medium, in a glass-bottomed culture dish (Matek Corp ,USA).

Spleens were dissociated in complete medium (αMEM (Life technologies), 10% serum (Biowest), 2 mM L-Glutamine, 100U/mL Penicillin, 100 µg/ml streptomycin (Invitrogen)), then filtered through a 100 µm cell strainer (Falcon).

Cells were dissociated in Ham-F10 medium (Invitrogen) with 0.025% Trypsin (Sigma) and centrifuged over a 4% (w/v) BSA cushion at 800 × g for 5 min.