Sentence examples for medium were isolated from inspiring English sources

The proteins, obtained from cells grown in LB medium, were isolated by osmotic shock in a 20 mM Tris, 5 mM dithiothreitol (DTT) buffer at pH 8.

HeLa cDNA-expressing plasmids from clones growing on high stringency medium were isolated and retransformed into competent yeast expressing BD-IncA (aa 121-383).

Briefly, cells cultured in M9 medium were isolated, and subsequently DNA was precipitated with ammonium sulfate.

After 14 days of cultivation, evolved strains that exhibited robust growth on the inhibitor-containing medium were isolated.

Mauve colonies on Chromagar, or colonies suggestive of S. aureus on blood or Baird-Parker medium were isolated and confirmed with Vitek II (bioMérieux, Nürtingen, Germany) using GP and AST-P592 cards for species identification and antimicrobial susceptibility testing, respectively.

At each identified concentration of TH and at different incubation times, 1 × 10 cells from culture medium were isolated, washed with ice cold phosphate buffer solution, resuspended in 100  μL binding buffer, and stained with 5  μL of FITC-conjugated Annexin V (10 mg/mL) and 10  μL of PI (50 mg/mL).

Twelve hours later, the medium was changed to the serum-starvation medium, and aggregates were isolated after 1 4 days of incubation.

Three-week-old calli inducted from seeds were transferred to fresh medium, and mitochondria were isolated after one week of incubation.

After the addition of double stranded RNAs targeting SPS1 to the culture medium, total RNAs were isolated on days 1, 3 and 5 after treatment and subjected to microarray analysis using Affymetrix microchips (GEO accession number: GSE 17685).

Cyclohexane-tolerant mutants that formed colonies on the LBGMg agar medium overlaid with cyclohexane were isolated from strain JA300.

Positive clones were grown in LB medium, and the plasmids were isolated using a miniprep alkaline lysis method [45].