Sentence examples for medium containing uracil from inspiring English sources

The difference in relative regulation between urg1, urg2, and urg3 seems to mainly reflect differences in basal expression levels: while all three genes show similar absolute expression signals in rich medium (containing uracil), the expression signals in minimal medium are ∼3- and 4-fold higher for urg2 and urg3, respectively, compared to urg1 based on Affymetrix chip data [19].

Isolated GIA39 DE3) transformants were streaked from minimal medium containing uracil onto minimal medium containing uracil or cytosine.

Cells lacking a functional CD will only grow on nonselective minimal medium containing uracil.

Similar to the URC genes, deletion of toe1 + and SPAC1399.04c prevented growth on medium containing uracil as the sole nitrogen source.

Loss-of-function alleles of the URC genes result in growth inhibition on medium containing uracil as the sole nitrogen source (Andersen et al. 2008).

Colonies resistant to 5-FOA were cultured in medium containing uracil for genomic DNA isolation and PCR screening of the targeted region.

leu− ura− cells transformed with the pSP1-meiURA4 plasmid grew on minimal medium (MM) plates containing uracil but not on plates without uracil.

When these two colonies were spread on minimum medium containing no uracil in order to maintain the selection pressure, only granular colonies developed.

Strains were grown for 2 days in a non-selective liquid medium, which contains uracil (YPD), to allow growth of cells that lost the plasmid containing the URA3 counterselectable marker (Boeke et al., 1984).

(B and D ) BY384 and tRNASerGCU quadruple deletion strains, respectively, grown on liquid medium that contains uracil (YPD) to allow the growth of cells that lost the plasmid and then plated on 5-fluoro-orotic acid (5-FOA) plates (see 'Materials and methods').

As a quick check to establish that each vector was capable of correcting the pyrE allele, all three were independently transformed into CRG1545 and plated on CGM medium containing 20 µg/ml uracil and 15 µg/ml Tm, and selected colonies restreaked onto CBM lacking uracil and any antibiotic supplementation.