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The cultivation of FY834 strain was carried out in YPD medium containing ethanol and then the culture was transferred to fresh medium containing the same ethanol concentration.
Medium containing ethanol alone was used as a control.
They were induced on medium containing ethanol and oleic acid as carbon source.
Fresh medium containing ethanol at 101.4 μL of 100% ethanol/100 mL of medium (80 mg/100 dL), FIV at MOI of 0.5, or ethanol (same dose) plus FIV (same dose) were added to these cultures for 24 hours.
As a comparison, cells were pre-treated with 10 μg·ml−1 BFA (dissolved in ethanol) or the vehicle solvent alone (0.5% ethanol), and then treated with dilutions of STx in growth medium containing ethanol or ethanol/BFA as appropriate.
When ampk and daf-18 mutants in L1 diapause are cultured in M9 medium containing ethanol, many surviving animals lack the overt tissue degeneration observed in the absence of ethanol.
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When the feed medium contained ethanol as the sole carbon source, the highest biomass yield and PHB yield on substrate, 0.45 Cmol/Cmol and 8.5 Cmmol/Cmol, was observed when the chemostat was operated at the dilution rate of 0.05 h-1.
Conclusions concerning a direct apoptosis induced by DEE might be too far-reaching, since no cytotoxicity was measured and the extracts were applied to the cells in a medium containing 90% ethanol (ethanol concentrations at and above 1% in the medium are cytotoxic).
Lloyd et al. found that yeast previously grown in the presence of 5% ethanol could grow in the medium containing 10% ethanol, whereas yeast inoculated directly into medium containing 10% ethanol failed to grow [2].
All formulations failed f2 in the presence of dissolution medium containing 40% ethanol when compared to 0% ethanol medium (Table 6).
After 3 d seedlings were carefully transferred to the same medium containing no ethanol (control medium) or to induction medium containing 0.1% (v/v) ethanol.
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