Sentence examples for medium lacking uracil from inspiring English sources

A functional Ura3 protein is produced, enabling selection on medium lacking uracil for the parental vector.

The cells were cultured on synthetic complete medium lacking uracil and tryptophan, either with histidine (SC-UW) or without histidine (SC-UWLH).

SH8836 strain was cultivated on SC solid medium lacking uracil and tryptophan, and then cells were replicated onto SC solid medium lacking tryptophan and containing 5-fuloroorotic acid (5-FOA) and uracil for curing pRRN5 plasmid.

Selection and cultivation of yeast transformants were conducted on Synthetic Dextrose (SD) medium lacking uracil (SD-URA plates: 6.9 g/L yeast nitrogen base (YNB) without amino acids (Formedium), 0.77 g/L complete supplement mixture without uracil (Formedium), 20 g/L glucose and 20 g/L agar).

Expression of URA3 is necessary to support growth on medium lacking uracil.

Culture of transformants was performed in liquid selective medium (lacking uracil).

Transformants were selected on SD medium lacking uracil and screened by PCR.

Transformants were selected on SD medium lacking uracil and leucine to confirm the successful transformation of the two constructs.

Cells with p316CupNGMC were grown in dextrose medium lacking uracil and diluted to OD600 nm  = 0.05 into the same medium and into identical medium with 25 µM CuSO4.

Silencing was stronger in these cells since they were unable to grow in minimal medium lacking uracil, whereas the control cells could grow.

Log phase cells containing pDCM90 grown at 30°C in dextrose medium lacking uracil were first diluted to OD600 nm  = 0.2 into the same medium.