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LN229 control cells or LN229 cells expressing myrAkt were cultured in serum-free medium containing glucose.
B. breve A1 was cultured in a medium containing glucose, yeast extract and salts.
The medium containing glucose was used as control.
The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.
As part of a larger experiment20, we propagated an initially isogenic population of P. fluorescens strain SBW25 for 1,000 generations in a minimal medium containing glucose as the sole carbon source.
As part of a larger experiment, we propagated an initially isogenic population of P. fluorescens SBW25-lacZ for 1,000 generations in a minimal medium containing glucose as a sole carbon source20.
(b) Glutamine was supplied where indicated (***P<0.005 compared to medium containing glucose but no glutamine; #P<0.05, ##P<0.01 compared to glucose- and glutamine-deprived medium; n=6).
(a) Lactate was supplied where indicated (**P<0.01, ***P<0.005 compared to medium containing glucose but no lactate; ##P<0.01 compared to glucose- and lactate-deprived medium; n=3).
The overexpression of the tpi2 gene restores the growth deficiency of tpi1 knockout mutant on the minimal medium containing glucose or glycerol.
The experiments were performed in a mineral salt medium containing glucose and sugars obtained from apple and carrot pomaces by acidic hydrolysis.
Optimization by mixture analysis resulted in a synthetic medium containing glucose:furfural ACN DMSO (1%, 2 mM, 0.2% and 0.3%, respectively).
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