After removing the plasmid complexes, the cells were post-incubated for 36 h in medium containing serum.
Furthermore, cells incubated with nanoparticles were washed by centrifugation and resuspended in medium containing serum up to 3 times.
This PBH-capped AuNP had the same hydrodynamic size distribution profile range (150 to 260 nm) in EMEM/S- as in a water suspension and in medium containing serum.
Cells were always cultured in medium containing serum.
Extraembryonic endoderm (XEN) cells were derived in medium containing serum without additional growth factors.
MCF-7 cells showed robust mTORC1 activation in complete medium containing serum and nutrients (Fig. 3 5).
Eight hundred µl of fresh medium containing serum was added 2 hours later followed by cells harvesting 48 hours later.
Mouse ES cells were routinely maintained on gelatin coated tissue culture plates in medium containing serum and LIF as described [31].
Eletroporated cells were resuspended in culture medium containing serum and supplements without antibiotics and incubated for 24 h at 37°C in a humidified 5% CO2 incubator.
The cells were rendered quiescent 24 hours later by serum starvation for a period lasting a further 24 hours, and then stimulated by adding medium containing serum.
By adding precipitating ions to cell culture medium containing serum, mineral nanoparticles are generated that are morphologically and chemically identical to the so-called NB.
