Sentence examples for medium containing methanol from inspiring English sources

The enrichment culture was cultivated in a bicarbonate-buffered medium containing methanol and perchlorate at 37°C.

Then, cells were diluted and plated on the solid Burkholder medium containing methanol (0.5%) and allyl alcohol (0.3 mM).

M. extorquens AM1 was grown in fed-batch mode in mineral medium containing methanol as sole carbon and energy source, as described in [ 19].

Four independent glucose-grown cultures were used to inoculate fresh medium containing methanol as well as for taking samples for RNA isolation.

Glucose cultures in the late exponential growth phase were transferred to fresh mineral medium containing methanol as sole carbon and energy source.

However, two hours after the shift to medium containing methanol, mRNAs of both genes were readily detected, a time-point which has also been identified as threshold for the detection of first AOX enzyme activity [ 5].

After vigorous overnight shaking at 30°C the cells were harvested by centrifugation and resuspended in the same medium containing 0.5% methanol in place of glycerol and shaken again for 24 h.

All subsequent growth was in medium containing just methanol.

A 2-day culture in a medium containing 0.5%% methanol resulted in optimal enzyme production.

For populations F1-F8, individual colonies were used to inoculate each flask as before, but this time the very first growth cycle was in medium containing both methanol and succinate.

Fitness assays utilized cultures that were first acclimated from the freezer for four days in medium containing both methanol and succinate at half their usual concentration (7.5 mM and 1.75 mM, respectively) in order to allow sufficient growth from the frozen stock.