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Previous research had demonstrated the formation of microemulsions in an aqueous medium containing methyl methacrylate (MMA) and acrylic acid (AA).
Carbon-limited (1.1 g/L cellobiose) chemostat grown cultures (0.1 h−1) were supplied with MTC medium containing methyl viologen (Fig. 1).
Time "0" for methyl viologen addition was the point when the feed medium was switched from MTC medium to MTC medium containing methyl viologen (~88 h after the experiment began).
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Following drug incubation for 24 h (geldanamycin, roscovitine and LY294002) or 30 min (U0126), cells were washed twice with PBS to remove drug and incubated with medium containing (methyl-C choline chloride (Amethyl-C cholinei per 7 chloride.
In the experiments with different endocytic inhibitors HeLa cells were preincubated in medium containing methyl-β-cyclodextrin (2.5 mM), nocodazole (60 μM), cytochalasin D (4 μM) or amiloride (0.4 mM) for 30 min, followed by TAT-SA-A488 transduction.
To test the various clones' response to UV light, cells suspended in 0.5 mL phosphate- buffered saline (PBS) were introduced into six-well plates and irradiated with UV (254 nm wavelength) at doses ranging from 0 to 9 J/m, followed by the addition of 5 mL of the complete medium containing methyl-cellulose.
CYE1 CMC Yeast Extract agar medium containing carboxyl methyl cellulose (CMC) as carbon source.
At 24 hpi, the cells were washed and incubated in fresh medium containing 1% methyl cellulose.
[ methyl-C]Methionine-labeled pol β(L22P) and pol β(L22P LD (residues 1 87) were prepared as described previously by growth of plasmid-containing Escherichia coli on a medium containing [ methyl-C]methionine (CIL, Cambridge, MA).
After 3 days of growth, surviving cultures were replicated to a medium containing 6-methyl purine.
Methylcellulose colony assays were performed in RPMI-1640 medium containing 1.1% methyl-cellulose (STEMCELL Technologies Inc., Vancouver, Canada) and 20% FBS.
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