Ai Feedback
Exact(16)
Cells were further incubated for 1 hour in medium containing gentamicin 100 µg/ml for elimination of extracellular bacteria (bacteria uptake).
The cells were washed twice with DMEM and then incubated for 2 h with the medium containing gentamicin (50 µg/ml).
After washing (time zero of the kinetic analysis), the cells were incubated in fresh culture medium containing gentamicin (10 µg ml−1) to kill extracellular bacteria.
The monolayers were then washed four times with PBS and fresh medium containing gentamicin (100 µg/ml) was added to kill extracellular bacteria (time zero of the assay).
After removal of gentamicin IPEC-J2 cells with intracellular Salmonella were incubated with E. coli for 2 hours and then incubated for up to 20 hours with medium containing gentamicin to kill extracellular bacteria.
Cells were washed 3× with PBS and fresh medium containing gentamicin (50 µg/ml) added.
Similar(44)
Heart and brain tissues from sentinel hamsters were triturated in Eagle's minimal essential medium (MEM) containing gentamicin and 20% fetal bovine serum to generate 10% suspensions.
The media was replaced after 1 h with medium containing 5 µgentamicintamicin.
Transformed seedlings were selected on solid MS medium containing either gentamicin or phosphinothricin and screened for GFP expression using a stereo fluorescence microscope.
To obtain the IP, 6 g biomaterial was added to 1 L low-glucose Dulbecco's modified Eagle's medium (Gibco, CA, USA) containing gentamicin (60 μg/L), penicillin (100 U/mL), streptomycin (100 μg/mL) and amphotericin (25 μg/mL) (PSA, Sigma-Aldrich, USA).
Primary dNeo-Neonatal human dermal microvascular endothelial cells (MVECs) were purchased from Lonza (Lonza Workingham Ltd ,Workingham, UK) and maintained in complete EGM-2MV Microvascular Endothelial Cell Growth Medium-2 (Lonza Workingham Ltd ,Workingham, UK) (containing gentamicin, 5% (v/v) FCS, hEGF, hydrocortisone, VEGF, hFGF-B, R-IGF-1 and ascorbic acid).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com