Sentence examples for media containing gentamicin from inspiring English sources

Cells were then washed with fresh media, and incubated for 45 min at 37 °C with media containing gentamicin (50 μg/mL) to kill any extracellular bacteria.

TER of monolayers was measured after switching to fresh media containing gentamicin to prevent further bacterial growth.

To enumerate intracellular bacteria, after washing with PBS, cells were further incubated in media containing gentamicin (100 µg/ml) for 1 hour, washed, lysed and dilution plated.

Infections were cleared by removing the media and washing cells 3 times in PBS, and adding media containing gentamicin and ciprofloxacin.

For the invasion assay, the infected monolayers were washed 3 times with MEM, fresh complete media containing gentamicin (100 µg/mL) was added and incubation for an additional 2 h was performed to kill extracellular bacteria.

For initial invasion studies, we infected the cells for 15 minutes prior to fixation, whereas for comet tail studies infections persisted for 30 minutes at which point the media was swapped with warm media containing gentamicin for 1 hour (initial comet tail formation) or 4 hours (established comet tail studies).

Cells were cultured in X-VIVO 10 media (Lonza) containing gentamicin and 1% autologous plasma.

A 10% homogenate in Earle's minimal essential media (MEM) containing gentamicin was made of each specimen.

After 4 h of co-culture, the cells were washed two times with PBS and the media replaced with fresh K-SFM containing gentamicin (200 µg mL−1) and further treated with caffeine, LY294002 or Z-VAD during the remaining time of the incubation.

About 4.3% of gentamicin present in the matrix was released from the samples containing gentamicin alone as compared to 18.6% for samples containing tobramycin alone.

Palacos cement (Zimmer Biomet Holdings, inc., Warsaw, Indiana, USA), loaded with Stimulan beads containing gentamicin, was inserted into the segmental defect.