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Electroporated cells were recovered in 10 ml of complete medium and analysed 48-h post transfection.

After several washes in PBS, the embryos were mounted in Vectashield Mounting Medium and analysed with a confocal microscope.

In a novel approach, we added different concentrations of glucose (repressor) to the medium and analysed its impact on recombinant protein produced.

Sections were covered with mounting medium and analysed with a light microscope.

The single colonies of the survivors were selected, grown on HS liquid medium and analysed by psbA gene sequencing.

Slides were air-dried at room temperature and coversliped with eukitt mounting medium and analysed on a Nikon microscope.

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Similar(46)

Samples were mounted in non-aqueous mounting medium and then analysed with a Bright field microscope.

The cells were incubated with hydroxyvitamin D3 derivatives for 2 h, followed by fixation with 4% paraformaldehyde and mounting with fluorescence mounting medium, and were analysed on a fluorescent microscope.

Therefore, the RfBP+ nfxl1 hybrid was identified based on growth in kanamycin-containing MS medium and PCR analyses of both RfBP and the insertion flanking sequence (Additional file 6: Table S1).

After 30 min the apical medium was discarded, whereas the basal medium was collected and analysed for p24 Ag concentration.

The amended and un-amended polybag medium was collected and analysed before planting and after harvesting.

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