Sentence examples for vector and analysed from inspiring English sources

Therefore, we cloned the −1091/+43 region of the human endosialin gene into the promoter-less pGL3-basic vector and analysed its activity in a dual luciferase reporter assay.

The cells were transfected as described above in 48-well plates using the pEGFP-N1 vector and analysed 48 h post-transfection using a FACS sorter (FACSCalibur, Beckton Dickinson, Oxford, UK) and Cell Quest software.

Cells transiently transfected with LC3 GFP alone, or co-transfected with empty vector and analysed 48 h after transfection, display few cytoplasmic LC3 GFP punctae (Fig.  1A and B).

To investigate whether the suppression of GnT-IVa expression in Jar could influence the invasive ability in vivo, we established a stable GnT-IVa knockdown model of choriocarcinoma, using an shRNA vector, and analysed the effects on tumorigenicity in nude mice.

Stained sections were mounted using Vectashield with DAPI (Vector) and analysed with a Leica DMI4000B/DFC 340FX inverted microscope.

The sections were counterstained with hemalun, mounted using an aqueous-based medium (Vectamount AQ, Vector), and analysed as described in Figure 3.

MTHA and VT cloned PMMs into GFP vectors and analysed their effects.

Organs were collected for stereoscopic imaging, immunohistochemistry, and vector and protein analyses.

Sections were mounted with Vectashield mounting medium with diamidino-2-phenylindole (DAPI) (Vector, Peterborough, UK) and analysed by fluorescent microscopy.

Next, we introduced the resulting plasmid pJEM- mptA2 and the promoter-less pJEM15 vector into MSMEG and analysed β-galactosidase activity of untreated or TPEN treated cultures.

Using COS7 cells transfected with a p94 expression vector and Western blot analyses, we previously detected proteolysis of several proteins, suggesting that p94 prote-olytic activity is readily exerted and that the proteins identified are potential in vivo p94 substrates [ 17].