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To examine and quantify the effects of glass beads and chemoattractant on bacterial motility in granular media, we examined the motile behavior of P. aeruginosa in a saturated granular medium and quantified the effects of glass beads and the presence of a chemoattractant.
We tested their capacity of performing phagocytosis vacuoles adding Indian ink to the medium and quantified the cells that presented food vacuoles containing ink (Figure 3A D).
The bacteria were resuspended in complete medium and quantified by densitometry (Densimat, Bio Mérieux) using McFarland standards (McFarland of 0.5 corresponds to approximately 1.5 × 108 bacteria/ml).
Bacteria were centrifuged at 10,000 rpm for 2 minutes, washed with BHI medium three times, reconstituted with BHI medium, and quantified by spectrophotometry.
The purified bacillary suspension was prepared in synthetic 7H9 Middlebrook broth-OADC medium (DIFCO, Detroit, MI, USA) (7H9-OADC medium) and quantified via a nephelometric reference curve prepared with known quantities of bacteria.
Briefly, the MTS dye, 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxymethoxyphenyl -2- 4-sulfo-phenyl -2H-tetrazolium, is converted to a formazan product that is soluble in tissue culture medium and quantified by its absorbance at 492 nm.
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Based on a central composite experimental design, the effects of five factors: pH (4.6 6.6), sodium chloride (NaCl, 0 10%), sodium nitrite (NaNO2, 0 152 ppm), pressure (P, 300 600 MPa) and pressure-holding time (t, 0 10 min) on the inactivation of Listeria monocytogenes by high hydrostatic pressure processing on a simulated meat medium (SMM) were evaluated and quantified.
Different concentrations of Aβ (1 42) (Bachem) aggregates were prepared according to the protocol by Klein [1] in Neurobasal medium without phenol red, and quantified using Immunoblots.
The attached cells were incubated with CCK-8 in medium for 4 hr and quantified by measuring the absorbance at 450 nm with a SpectraMax 190 microplate reader (Molecular Devices, Sunnyvale, CA, USA).
Gels were stained with 0.75 μg/mL ethidium bromide for 30 min. DNA bands were visualized with medium-range ultraviolet light and quantified using an Alpha Innotech digital imaging system.
We manipulated the abundance of flies in the nests of medium ground finches (Geospiza fortis) and quantified the impact of the parasites on nestling growth and fledging success.
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