Sentence examples for medium and assayed from inspiring English sources

After one HBSS rinse, the adherent Sertoli cells were recovered for 48 h in the enriched DMEM-F12 medium and assayed by RT-PCR amplification for the expression of the germ cell marker c-Kit.

To determine whether non-α-DG glycoproteins hyperglycosylated by LARGE overexpression promote laminin assembly at the cell surface, we added laminin to the cell culture medium and assayed for bound laminin by immunofluorescence staining.

Briefly, cells were seeded in 96-well plates at a density of 1.5 × 10 cells/well in 100 μL of medium and assayed for proliferation after 48 h.

The cells were seeded in six-well dishes with 2 ml of culture medium and assayed at the indicated time by Western blotting and FACS.

Jurkat cells in exponential growth were electroporated (20  μg DNA, 270 V 975  μF) in a Gene Pulser (Bio-Rad, Berkeley, CA, USA), transferred to complete medium and assayed after 24 h.

Under method 2, cells were cultured in 96-well plates in serum-free medium and assayed for viable cell growth using the Cell Counting Kit-8 (CCK-8) from Dojindo Molecular Technologies, Inc. (Rockville, MD).

Conversely, El-Hadi et al. (2013) also reported that a maximum CMCase production of 0.23 U/mL was achieved at pH 7.0 in a liquid medium and assay temperature of 37 °C for Aspergillus hortai.

This culture was maintained by replacing the culture medium and assaying for sub-genomic DENV RNA every seven days.

To confirm the validity of this tracer assay, 15 h prior to and during the PPP assay, 6-AN (6-aminonicotinamide; 500 μmol/l), a competitive inhibitor of G6PDH, was added to the nutrient medium and assay solution, and the PPP activity was measured.

After 18 h, the medium was collected and assayed by using Gaussia-Dura Luciferase Glow Assay Kit (Pierce).

This culture medium was collected and assayed for monocyte chemotactic activity using chambers purchased from Neuroprobe (Cabin John, MD).