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Cell proliferation is measured as a function of changing electrical impedance as per the manufacturer's instruction and analysed using RTCA software (Roche).
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Briefly, 5 × 104 HSCs were stained with phycoerythrin-conjugated CD34 (CD34-PE) and fluorescein isothiocyanate-conjugated CD45 (CD45-FITC) (Biosciencesces, USA) according to the manufacturer's instructions and analysed.
For quantitative RT-PCR, the PCR reaction was conducted using the Absolute SYBR Green Rox mix kit (ABgene) according to manufacturers instructions and analysed with the DNA engine Opticon™ software (MJ Research).
Cells were labelled after treatments according to the manufacturer's instructions and analysed by flow cytometry.
Cells were prepared according to manual instructions and analysed by flow cytometry.
The microarray slides were processed according to manufacturer's instructions and analysed using Codelink System Software GE Healthcare Bio-Sciencess).
Sequencing was performed with BigDye v3.1 (Applied Biosystems) following the manufacturer's instructions and analysed on a 3500Dx Genetic Analyzer (Applied Biosystems).
Both tests were performed according to the manufacturers' instructions and analysed without knowledge of Lp 1 infection status.
Transient transfections were performed with Lipofectamine 2000 (Invitrogen), Fugene HD (Roche) or according to the manufacturer's instructions and analysed after 24 or 48 h.
The PCR products were sequenced using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit v3.1 (Applied Biosystems, California, USA) according to manufacturer's instructions, and analysed on an ABI PRISM 3100- Avant Genetic Analyzer (Applied Biosystems).
Keratinocytes lysates were assayed using the Promega dual luciferase reporter assay kit according to the manufacturer's instructions and analysed on a Glomax luminometer (Promega).
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