Sentence examples for media lacking serum from inspiring English sources

The cells were then incubated for 7 h, after which media was replaced with fresh media lacking serum.

Cell treatments were completed using media lacking serum and containing either 1 μg/mL hydrocortisone (HC) (Sigma), 10 μM RU-486 (Sigma), or ethanol vehicle for 48 hours.

All drugs were diluted to a 2× final concentration in 70%L-15L-15dia lacking serum and penicillin/streptomycin before being added to cells.

Briefly, THP-1 cells grown on non-TC-treated plates were washed and resuspended to 5 10 × 10 cells/ml in TC media lacking serum.

We also observed that the presence of serum in culture media modestly decreased (∼2-fold reduction in media containing 50% serum compared with media lacking serum) but did not abrogate the delivery of +36 GFP-mCherry into cells (Supplementary Figure 5).

We therefore grew each of the cell lines to approximately 80% confluency under non-limiting conditions, then changed to media lacking either serum (Figure 3a) or glutamine (Figure 3b), and subsequently assessed viability.

Survival was assessed by culturing OPCs in basic media lacking both serum and growth factors (DMEM (Gibco 41966-029)) for 24 h in the presence of each of the four types of NP by TUNEL assay (Invitrogen Click-iT TUNEL Alexa Fluor Imaging Assay).

For serum stimulation, cells were incubated in media that lacked serum for 24 hours (h), and then either unstimulated, or stimulated for 1 h with media containing 10% fetal calf serum (FCS).

This is achieved by the use of a well defined basal media that lacks serum.

The results obtained in this single-center experiment confirm that the serum-free media used in the proficiency panel lead to background reactivity which was comparable to the background spot production induced by freezing media containing human AB serum, but also to other freezing preparations containing or lacking serum (FCS or CTL Cryomedium, respectively).

Native CST1-His and CST3-His proteins were purified from conditioned media lacking fetal bovine serum (FBS), and their involvement in cellular invasion was examined.