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In contrast, PexoT-lacZ transcription in PAO1 was induced in media lacking calcium.
The experiments were performed in the presence and absence of calcium, since minimal media lacking calcium induces expression of T3SS gene expression [35].
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The calcium chelator BAPTA exacerbates the poor growth of halA− sltA− strains on media lacking added Ca2+ (Fig. 1A).
Transformants were selected on SC media lacking tryptophan and leucine.
Positive transformants were selected in SC media lacking Trp.
Unlike VDH1, VdGARP1 accumulation is greatly induced in media lacking glucose but inhibited in media lacking nitrate (Figure 5A).
Transformants were replica-plated onto media lacking leucine or YPD media containing geneticin.
Control cultures received media lacking norepinephrine.
The cells were then incubated for 7 h, after which media was replaced with fresh media lacking serum.
We tested spheroid budding by replacing serum-containing media with spheroid base media lacking these added growth factors.
AML1 fragments were cloned into pGBKT7, singly transformed into AH109 yeast cells, and assayed for growth on media lacking tryptophan (to confirm transformation) and media additionally lacking adenine.
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