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Somewhat surprisingly, media lacking glucose did not induce XBP-1(S) in either cell line.

Unlike VDH1, VdGARP1 accumulation is greatly induced in media lacking glucose but inhibited in media lacking nitrate (Figure 5A).

Follow this, cells were collected by brief centrifugation, washed in media lacking glucose, briefly centrifuged and re-suspended for assessment by microscopy.

For glucose deprivation, cells were re-suspended in media lacking glucose and incubated in a flask at 30°C with shaking for ten minutes.

Melanized and non-melanized (tricyclazole-treated) C. sphaerospermum were grown on BBL Sabouraud dextrose agar for 15 days, harvested and plated on water agar prepared by mixing agar powder with minimal media lacking glucose.

Five mL volumes of sucrose-containing agar (prepared by mixing agar powder with 100 mg/L sucrose as described in [6] and with minimal media lacking glucose) was added to the right two sections of each plate.

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Similar(47)

The washing media lacked glucose and supplements, and MgSO4 was substituted by equal amounts of MgCl2.

Selective media lacking adenine and containing glucose as carbon source were used for selection of ADE+ colonies.

The transformed yeast were incubated on glucose media lacking leucine (Fig. 3A), galactose media which induces expression of the respective ANT genes (Fig. 3B), and media containing a nonfermentable carbon source (Fig. 3C).

Measurement of neural mitochondrial membrane potential from 30- to 35-day-old flies was conducted as previously described (Burman et al., 2012), except that dissections were performed in supplemented DME/Ham's F-12 High Glucose media lacking phenol red (Sigma), and all incubation steps were carried out at 25°C.

Log phase cells were normalized and plated on media lacking uracil and containing either glucose or galactose as the carbon source.

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