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Conversely, neither CUS2 deletion (Figure 4A) nor overexpression (Figure 4B) restored growth on media lacking histidine.
This allows the yeast strain carrying the NHR-64 fusion protein to grow on selective media lacking histidine.
Similarly, the U2 snRNA mutants did not grow on media lacking histidine and did not exhibit an Spt− phenotype similar to mutations in BUR2 (Figure 4C).
Of an estimated 2.5×106 transformants, 30 clones proliferated on media lacking histidine and adenine as well as stained positive for β-galactosidase.
Colonies were picked and re-streaked on selective media lacking histidine, leucine and tryptophan (-HLW), supplemented with 2 mM 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole
Sporulation of resultant diploid cells, followed by selection in media lacking histidine, led to the isolation of the haploid strain BYΔyer067wΔyil057c MATa, harboring both the KANMX4 and NATMX resistance cassettes.
Similar(27)
Cells co-transformed with two-hybrid constructs encoding proteins that interact will grow on media additionally lacking histidine and adenine (selective media).
After growth on 5-fluoro-orotic acid media also lacking histidine (to maintain selection for pRS423 based plasmids), cells were placed onto YPD to assess color and –ADE –HIS medium to assess the ability to grow on medium lacking adenine.
We observed a strong interaction between wRAR1 and wSGT1, which was confirmed in media lacking both histidine (-H) and adenine (-A), that results in a more stringent selection pressure.
Two-hybrid protein interactions were evaluated by growing the yeast colonies at 28°C on media lacking either histidine or adenine and supplemented with different amounts of 3-amino-1,2,4-triazole (3-AT).
Synthetic media lacking lysine or lacking histidine was used to prepare 1 liter of cells at 2.0 × 10 cells/ml.
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media lacking calcium
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media lacking lysine
media lacking growth
media lacking serum
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