Sentence examples for media lacking uracil from inspiring English sources

Transformants were selected for uracil prototrophy by plating on synthetic media lacking uracil (SC-Ura−).

The yeast strains used in this assay were transformed with the URA3 CEN plasmid pRS316 or a URA3+ 2μ plasmid, and selected on synthetic complete media lacking uracil.

These strains were grown in synthetic media lacking uracil or tryptophan, respectively, for plasmid selection with either 5% glucose (repressed) or 0.05% glucose (derepressed).

Log phase cells were normalized and plated on media lacking uracil and containing either glucose or galactose as the carbon source.

Absence of growth on minimal media lacking uracil indicates insufficient cytosolic Ura3p levels, which may result from improper protein synthesis or transport.

S. cerevisiae 3MeA DNA glycosylase (MAG) was expressed in yeast when galactose, at final concentration of 2%, was added to SC drop-out media lacking uracil (Q-BioGene, Carlsbad, CA).

Transformants were grown in synthetic complete (SC) media plates lacking uracil, tryptophan, and histidine at 30°C to a concentration of 5×106 cells/ml.

Cotransformants were assayed for growth on selective media either lacking uracil (-URA) or histidine (-HIS), in addition to assaying the production of β-galactosidase (X-GAL).

Synthetic dropout (SD) solid media appropriately lacking uracil or amino acids were used for selection of yeast plasmid transformants.

(B ) Growth curves in selective, synthetic liquid media (YNB lacking uracil and containing 0.05% casamino acids and 2% dextrose, with or without 1 mM ZnCl2).

The transformants were selected on synthetic minimal media supplemented with glucose but lacking uracil (SD-URA) and then patched onto rectangular agar plates (OmniTray; Nunc International).