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Medium lacking serum was termed 'basal medium' (BM), while medium containing 10% FBS was termed as "full medium" (FM).
To examine whether perhexiline, niclosamide, amiodarone and rottlerin inhibited the amino acid-dependent phosphorylation of Thr37/46, MCF-7 cells were exposed to perhexiline, rottlerin, amiodarone or niclosamide in medium lacking serum for 1 h or 4 h.
Subconfluent cells were grown overnight in medium lacking serum.
The cells were then washed twice, and given medium lacking serum.
For serum starvation, the supplemented medium was replaced with medium lacking serum.
The resulting cells can be perpetuated in defined medium lacking serum products or growth factors.
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HeLa cells were grown in a medium containing or lacking serum for 24 h, after which the drugs were administered for an additional 72 h under the same conditions.
S37P-hTERT cells were impaired in their motility both in normal growth medium and particularly when culturing cells in poor medium (medium lacking either serum or l-glutamine).
Two hours before running the assay, control cultures were transferred to Seahorse XF assay medium with GlutaMax and high glucose (at 4500 mg/L) but lacking serum and sodium bicarbonate.
When cultures reached approximately 80% confluence in 15-cm culture dishes, medium was replaced with fresh basic MSC culture medium (see above in Multipotent stromal cell isolation and culturing methods) either with 10% FBS or lacking serum.
Serum starvation was carried out by incubating cells in their corresponding medium lacking fetal bovine serum.
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