Matrix pH was superimposable in WT and Opa1tg MAFs, irrespective of whether the cells were cultured in media containing glucose or galactose (to force mitochondrial ATP production, Supplementary Fig. 1e).
Filamentous fungi were grown in potato dextrose broth (PDB) containing glucose 20.0 g/L or modified Czapek-Dox media containing glucose 20.0 g/L; NaNO3 3.0 g/L; K2HPO4 1.0 g/L; MgSO4·7H2O 0.5 g/L; KCl 0.5 g/L; FeSO4·7H2O 0.01 g/L in distilled water and the pH was set to 7.3.
These strains were grown in the presence of glucose, then washed and re-suspended at OD = 0.5 in MH media containing glucose or arabinose, then spotted directly on glucose or arabinose containing media (0h) or after 1 h incubation at 37 °C with arabinose (1h).
In the present investigation, when mutant Mut-4 was cultivated in optimized fermentation media containing glucose and defatted cottonseed meal, bikaverin as well as gibberellic acid production was first observed at 24 h when culture entered the stationary phase.
Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize novel medium chain-length polyhydroxyalkanoates (mcl-PHAs), which accumulated 22% - 56% of cell dry weight when cultured, under batch culture conditions in media containing glucose, waste vegetable fryer oils or fatty acids (Sharma et al. [2011]).
Similar results were obtained in media containing glucose and 0.85M NaCl (not shown).
Bax−/−/Bak−/− MEFs are protected against DETA-NO in media containing glucose (Figure 1A D).
Pelleted cells were resuspended in distilled water and serially diluted before eIF4E-dependent translation complementation was assessed on selective drop-out media containing glucose.
Spotted cells were grown either for 5 days on media containing glucose, or for 9 days on media containing glycerol/ethanol either with or without BCS.
Unless otherwise indicated, yeast cells were grown at 30°C in minimal media containing glucose (SD) to late log phase (A600∼1.0), washed with ice-cold H2O and resuspended in ice-cold 2% glucose.
