Sentence examples for medium lacking glucose from inspiring English sources
We isolated swarmer cells and incubated them in M2 medium lacking glucose for 15 min before collecting samples (Figure 3A).
Incubation of MEFs with DPBS (medium lacking glucose, amino acids and serum) for 4 h resulted in a small reduction in cell number at 48 h.
Figure 5A shows that accumulation of VdGARP1 mRNA increased by incubation with sorbitol and NaCl, as well as in a medium lacking glucose (Figure 5A).
For this purpose, a patch of colony on agar medium was picked to culture in liquid medium for three days; different stress conditions were then applied by adding sorbitol or NaCl, or by transfer to liquid medium lacking glucose or nitrate, and the cells were cultured for an additional eight hours.
At time point 0, the medium was changed to synthetic medium lacking glucose (red curves).
Furthermore, this proportion increases when cells are transferred into medium lacking glucose.
At the same time, OLs cultured under metabolic stress condition were transferred in to Seahorse XF assay medium with GlutaMax but lacking glucose, serum and sodium bicarbonate.
Cells lacking glucose produced little pyruvate in vitro but when glucose was available in the medium, there was a significant (P < 0.001) dose-responsive decrease in overall pyruvate production; 3.5 ± 0.1 nmol/l/h/1 × 10 viable GCs at 3 mM glucose compared with 2.5 ± 0.1 nmol/l/h/1 × 10 viable GCs at 6 mM glucose in the medium.
The transformants were selected on minimal glucose medium lacking uracil to select for the plasmids and their growth were tested on non fermentable medium containing 2% glycerol and 2% ethanol at 28 or 36°C.
As shown in Figure 3B, H4-R36A cells had modest growth on medium lacking histidine and containing glucose and much stronger growth on medium lacking histidine and containing galactose, indicating that cryptic initiation occurs in H4-R36A cells and that this defect is much more pronounced in the context of elevated levels of transcription.
All strains were cultured in SD medium lacking uracil and containing 2% glucose.
