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P-Akt was induced significantly from 5 to 15 min after LPA stimulation and maximal activation was at 5 min. P-Akt abundance returned to basal levels 15 min later (Fig. 4A).

This is in line with previous studies on different types of fatigue such as a prolonged (∼ 400 min) mountain marathon [42] or eccentric/stretch-shortening cycle exercise (e.g. [22]) where maximal activation was found to be back to baseline values as soon as one day post-exercise.

The maximal activation was achieved after 10 min, followed by a rapid decline in phosphorylated ERK1/2.

Thereafter, the stimulation current was increased in five to seven steps until maximal activation was reached, which was until no further increase of torque in response to triplet-stimulation occurred.

ROI-specific activation latencies were determined by measuring the point in time where 50% of the local maximal activation was reached for the first time (latency of half maximum).

Accordingly, the potential of its maximal activation was not different between control (–41.4 ± 1.4 mV, n = 7) and epileptic rat recordings (–37 ± 2 mV, n = 5, P > 0.05), and the maximal amplitude also did not show any significant change (111.1 ± 25.7 pA, n = 7, in control and 112 ± 10.4 pA, n = 5, in epileptic rat recordings, P > 0.05).

Assuming a selective stimulation of tibialis anterior accounting for 60% of force-generating capacity of dorsiflexors, a quarter of its maximal activation is required to produce dorsiflexion torque corresponding to 15%MVC.

The transcription rate upon maximal activation is given by V1,2 and corresponds to 33 transcripts produced per hour.

The Km and Vmax of ClpD in the absence of ClpT1 were 19.1 mM and 0.19 nmol/(min × μg protein), and those of ClpD in the presence of ClpT1 at a 1 1 molar ratio (maximal activation) were 28.3 mM and 0.43 nmol/(min × μg protein) respectively.

Features that were ranked high in our feature selection procedure were the range within the pattern, a feature based on the simplicity of a source separation, features that analyzed the spatial frequency and a binary feature which indicates if the maximal activation is on the border of the pattern.

Additionally, the pressure of half-maximal activation was shifted by ~9 mmHg towards lower pressures (P50 = −16.8 ± 2.8 mmHg with a 0 mmHg prepulse and P50 = −7.7 ± 1.1 mmHg with a +5 mmHg prepulse; N=11; P<0.05).