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Pfvap1 is expressed in ring stages with timing similar to PfEMP1; maximal expression was detected in highly synchronized ring stage cultures by quantitative real-time polymerase chain reaction (qRT-PCR; Fig. 1D) confirming previous reports (Le Roch et al., 2003; Otto et al., 2010).
TIMP1 (collagenase inhibitor) maximal expression was observed at day 7 for the hydrogel and ES-patch (16 and 25 fold respectively) and at day 14 for the D-patch (7 fold, Fig. 6j).
The GUS staining results showed that the expression of OsLAP6/OsPKS1 was only detectable in developing anthers (Fig. b-f); and the maximal expression was observed within the anthers at stage 9 (Fig. 5e).
The maximal expression was reached at day 7 (Fig. 8A).
Maximal expression was achieved at 48 h post transfection and the transfection efficiency was approximately 40% as detected by green fluorescent protein (GFP) under microscopic observations.
Only in 2 instances during growth did we detect anything higher than these values (32 36 h, and 48 52 h), and even then maximal expression was only 19 Miller units.
Similar(41)
Its maximal expression is concomitant with the peak emission of monoterpenes at the full-bloom stage (day 5 post anthesis, D + 5) (Hsiao et al. 2008).
In this system, the behavior near half maximal expression is biologically most relevant, since it is at this very concentration that the system has to 'decide' where to draw a fairly sharp gene expression boundary as one of the first steps in constructing a spatial pattern.
Treatment of these cells with doxycycline (Dox) potently upregulates p53 with maximal expression being reached by 24 h.
The data of the average expression levels is shown in Fig. 3 and that of the maximal expression is shown in Additional file 1.
For each gene model, the growth stage and the plant organ which showed maximal expression were ascertained and maximal expression profiles for growth stage and plant organs relative to the Arabidopsis gene models were constructed.
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