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Thus, the further increase in fluorescence intensity was unexpected, as full activation was anticipated to be independent of PI addition.
Aurora kinase B full activation was not required for H3 phosphorylation, this fits with the observations of Xu and collaborators (Xu et al., 2009).
In particular, the observed on/off switch may have significant advantages, especially considering that full activation was achieved at suboptimal Dox concentrations and thus might help to overcome induction problems related to tissue-specific barriers for effector penetration.
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Full activation is achieved by phosphorylation of Tyr416 in the activation loop.
Full activation is achieved through cleavage of a pro-domain generally by other caspases.
Full activation is achieved upon phosphorylation of S473 (Warfel et al., 2011, Mahajan and Mahajan, 2012).
Although the NRF asserts a basal level of activation through enhancements in ADP release, full activation is achieved through an NRF RS2 connection.
Recently, by incorporating mutant INCENP, Xu et al. revealed that the full activation is at least required for transferring the CPC to the midzone at anaphase onset (Xu et al., 2009).
Abatacept, the first of a new class of agents for the treatment of RA that selectively modulates the co-stimulatory signal required for full T-cell activation, was evaluated for its ability to regulate human T-cell proliferation and cytokine production in vitro.
Here full transcriptional activation is a good approximation, A >> K A and A >> K R, and the Hill functions become 1.
In fact, for the positive-feedback loops that form an HNC to be able to 'drive' the circuit towards full activation, it is necessary that sufficient activity is captured by them so that the amount of self-generated excitation overcomes the amount of 'leakage' and dispersion.
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