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Shh-stimulated migration of monocytes from CTR measured 172.5 ± 90% and a maximal stimulation was observed at Shh concentration of 1 μg/ml.
A maximal stimulation was observed for NaCl, whereas an intermediate level of stimulation was obtained for (Na)Hepes and (NMG Cl.
Maximal stimulation was reached after applying 25 μg/kg estrogen.
Maximal stimulation was obtained in three out of four tested cell lines at a concentration of 1 ng ml−1.
The maximal stimulation was again seen at 10 ng/ml of IL-1β, which produced a 22 ± 5 % increase in total cell number.
The concentration at which TIMP-1 caused maximal stimulation was 4 n M, in agreement with previous studies (Hayakawa et al, 1992, 1994; Yamashita et al, 1996).
Similar(49)
More broadly, however, the focus on minimal versus maximal stimulation is perhaps besides the point; the real goal of treatment should be "optimal" stimulation, an approach that requires tailoring the treatment plan to each individual couple.
Maximal stimulation is reached after 4 hours both by simulation and experiment.
The EC50 values (concentrations leading to half maximal stimulation) were around 6 × 10−5 and 2 × 10−12 for clodronate and E2, respectively.
The current required for supra-maximal stimulation was assessed by administering single pulses at rest.
First, a single super-maximal stimulation was given to verify the ability to detect a compound muscle action potential (CMAP).
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