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In the CatWalk test, morphine was effective one, two and six weeks after collagenase injection and maximal effect was observed from 60 to 90 minutes.
Taken together, both cohorts experienced interference from dexamethasone at a dose near 4.0 mg per day and a maximal effect was observed near 7.5 mg per day.
The maximal effect was obtained at a 25-μM concentration which is similar to concentrations of PPF used clinically.
Compounds were washed out once the maximal effect was observed.
The maximal effect was observed 7 days after the last MPTP injection (data not shown).
A maximal effect was achieved at ATP or ADP to DAPK ratios higher than two, which led to a Kd value of the probe of 6.40±0.54 µM.
The dose effect relationship (data not shown) revealed that maximal effect was observed at a 10 nM concentration for compounds 6g and 6h.
The results showed that triterpenes-induced JNK activation was abrogated by catalase in a dose-dependent manner and the maximal effect was obtained at 500 U/ml.
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However, localized plasmonic resonances have certain peak widths due to damping; the maximal effect is also believed to occur when the emission or excitation peak overlaps closely with the plasmon resonance peak [2].
A maximal effect is yielded by blocking the signalling pathways downstream of the receptor.
An EC50 value is that plasma concentration at which a half maximal effect is observed [ 25].
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