Further enzymatic analysis of β-glucosidase GH1-1 activity revealed that its maximal rate for GX cleavage was three times lower and its KM for GX was seven-fold higher than that for cellobiose (Table 3, Additional file 1: Figure S5).
The number of maximal missed cleavages was set to 1. Clone DKFZp686O1010Q (AccNo AL832139) containing A2ML1 full length cDNA was purchased from ImaGenes (Germany).
The chimaeric MNV* constructs and controls were co-transfected into HEK-293T cells with ptTA, a plasmid expressing the tetracycline trans-activator allowing for maximal promoter activity, and NS protein cleavage was assayed by Western blot.
For proteolytic cleavages, only tryptic cleavage was allowed, and the number of maximal internal (missed) cleavage sites was set to two.
For proteolytic cleavages, only tryptic cleavage was allowed, and the number of maximal internal (missed) cleavage sites was set to 2. Variable modifications of cysteine with carboxyamidomethylation, methionine with oxidation, and asparagine/glutamine with deamidation were allowed.
Low cleavage was still a daring thrill.
Cleavage was appreciated.
Remember when cars and cleavage was enough?
"I thought my cleavage was popping out.
PARP cleavage was detected by Western blotting.
