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These studies indicated that [11C]L-deprenyl-D2 can be used as in vivo marker for reactive astrocytosis, providing information concerning processes leading to neuronal loss.
At 100 days of age, both GFAP immunostaining, a marker for reactive gliosis, and ubiquitin immunostaining, a marker for protein aggregation, were significantly reduced in the treated mice, compared to the non-treated mice (Fig. 7B).
By using an antibody against GFAP, a marker for reactive gliosis in Müller cells, we could clearly demonstrate a dramatic increase in GFAP expression in β2−/−/γ3−/− mice (Fig. 1B') indicating that Müller cells were undergoing gliosis, a pathobiologic response, in β2−/−/γ3−/− retina.
GFAP, a marker for reactive gliosis, reflects Muller cell activation caused by RD-induced ischemia [ 38].
The technology is based on 5- and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, a reliable fluorogenic marker for reactive oxygen species in live cells.
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Neurons were also examined for nitrotyrosine (NT) immunoreactivity, a marker of reactive nitrogen species (RNS) formation.
An increase in oxidative stress proteins is an indirect marker for production of reactive oxygen species (ROS) [ 54].
We also determined the levels of microglia activation by using immunostaining for reactive microglial marker ED1.
They also found that the novices had levels of a marker for inflammation called C-reactive protein -- it's also associated with heart disease -- that was five times higher than that of the yoga experts.
19 A small (N = 13) study of healthy males designed to determine the body's response to the stress of hypoglycemia found an increase in proinflammatory cytokines, markers for lipid peroxidation, reactive oxygen species, and leukocytosis.
In addition to neoplastic glial cells which harbour the IDH1 mutation (Fig. 1K) as identified by double positive cells of mIDH1R132H and glial fibrillary acidic protein (GFAP, the classical marker for astrocytes), tumor samples generally contain reactive glial cells, vascular cells and phagocytic elements such as macrophages and microglia.
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