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We identified many mutant lines with reduced resistance to M. oryzae containing avrPish.
Among the many mutant lines screened by this group was an EMS induced mutation of AtMTP5.
This is surprising since many mutant lines are known in which egg-laying is reduced.
We additionally sequenced the accessions WS and Col-0, used as genetic background for many mutant lines and for over-expression lines of CBF genes [ 4, 22, 23].
For instance, many mutant lines analyzed by large-scale phenotyping initiatives show more than one phenotype annotation (Tang et al., 2010; Brown and Moore, 2012; White et al., 2013).
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There are also many useful mutant lines of Jurkat E6.1 T cells, each lacking a specific molecule in the peripheral TCR signaling pathway.
"We are hoping that 24-hour monitoring will allow us to see subtle changes to the behavior, feeding patterns, activity, and social interactions of many of our mutant lines," says Sara Wells, director of the Mary Lyon Centre at MRC Harwell.
Many of the mutant lines demonstrated small alterations in rigidity of the infected erythrocyte suggesting a large number of proteins potentially have a minor effect on this host cell property.
Additionally, large-scale mutagenesis projects and targeted mutagenesis technologies, such as zinc finger nucleases (ZFN) (Meng et al., 2008), transcription activator-like effector nucleases (TALEN) (Huang et al., 2011) and clustered regularly interspaced short palindromic repeats (CRISPR) (Hwang et al., 2013), are generating many new zebrafish mutant lines.
Thus, many mutants or transgenic lines of A. thaliana were shown to display differential levels of NaCl tolerance and this mostly concerned genes involved in ion transport [ 4- 8], detoxication processes [ 9, 10] or metabolite biosynthesis [ 11, 12].
In many cases, a gene had multiple insertional mutant lines, but the phenotypic effect was the same for all lines.
Related(20)
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